伍晓林,侯兆伟,杨二龙,金锐,刘俊豪,刘音颂
WU Xiaolin,HOU Zhaowei,YANG Erlong,JIN Rui,LIU Junhao,LIU Yinsong

• 1:多资源协同陆相页岩油绿色开采全国重点实验室
• 2:中国石油大庆油田有限责任公司
• 3:中国石油大庆油田有限责任公司勘探开发研究院
• 4:提高油气采收率教育部重点实验室(东北石油大学)

摘要(Abstract)：

为解析大庆油田低未熟陆相页岩储层微生物群落结构特征，探究其多样性、组成及潜在功能，采用高通量测序方法，对古龙凹陷和朝阳沟阶地的2个页岩岩心样本（YJY01、YJY02）进行微生物群落分析，通过Alpha多样性指数、物种注释、热图、Venn图等系统比较其群落结构差异。结果表明：2个岩心样本在门水平上优势菌群相似，主要包括变形菌门、厚壁菌门、放线菌门、拟杆菌门和绿弯菌门；但在属水平上差异显著；与样品YJY02相比，样品YJY01的丰富度和均匀度更高，群落结构相对更复杂；低未熟陆相页岩油储层中存在丰富且多样的内源微生物群落，其中包含多种具有原油降解、生物表面活性剂生成和储层改造潜力的功能菌属，具备微生物改质的应用基础。研究成果可为低未熟陆相页岩油开发提供新思路。
In order to analyze the characteristics of microbial community structure in Daqing Oilfield low-immature continental shale reservoirs and explore their diversity, composition and potential functions, high-throughput sequencing method is used to analyze microbial communities through 2 shale core samples(YJY01 and YJY02) from Gulong Sag and Chaoyanggou terrace. Systematic comparison of community structure differences are conducted by using Alpha diversity index, species annotation, heatmap and Venn diagram. The results show that, dominant bacterial communities of 2 core samples are similar at phylum level, mainly consisting of Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes and Chloroflexia. But there is a significant difference at genus level. Compared to sample YJY02, sample YJY01 has higher richness and evenness, with relatively complex community structure. Low-immature continental shale reservoirs are characterized by rich and diverse endogenous microbial community, including various functional bacterial genera with oil degradation, biosurfactant generation, and reservoir modification potential, being of application basis for microbial upgrading. The research provides new approach for the development of low-immature continental shale oil.

关键词(KeyWords)： 低未熟页岩;储层微生物;群落结构;生物降解;原位改质
low-immature shale;reservoir microorganisms;community structure;biodegradation;in-situ upgrading

Abstract:

Keywords:

基金项目(Foundation): 国家科技重大专项“泥纹型页岩油提高采收率技术研究及试验”（2024ZD1404905）

作者(Author): 伍晓林,侯兆伟,杨二龙,金锐,刘俊豪,刘音颂
WU Xiaolin,HOU Zhaowei,YANG Erlong,JIN Rui,LIU Junhao,LIU Yinsong

DOI: 10.19597/J.ISSN.1000-3754.202509041

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